The Need: In today's world, rapid and accurate detection of bacterial pathogens is crucial for safeguarding public health and animal well-being. With the emergence of diseases transmitted by ticks and the increasing importance of understanding vector-borne infections, there's a pressing need for efficient methods to identify bacteria like Ehrlichia chaffeensis.

The Technology: Our cutting-edge technology involves the development of a transposon mutant of Ehrlichia chaffeensis engineered to express mCherry (red) fluorescence, facilitating its easy detection in ticks, animals, and various cells. This innovation entails the precise insertion of the mCherry gene into the genome of E. chaffeensis, followed by the selection of this mutant strain using antibiotics. Through this method, we enable straightforward and reliable identification of E. chaffeensis, streamlining research and diagnostic processes.

Commercial Applications:

• Veterinary diagnostics for identifying Ehrlichia chaffeensis infections in companion animals and livestock.
• Public health surveillance to monitor the prevalence and spread of Ehrlichia chaffeensis in tick populations.
• Biomedical research applications for studying the pathogenesis and transmission dynamics of Ehrlichia chaffeensis.

Benefits/Advantages:

• Enhanced efficiency: Rapid detection of Ehrlichia chaffeensis simplifies screening procedures, saving time and resources.
• Accurate identification: The use of mCherry fluorescence ensures precise and reliable detection of the bacteria.
• Versatile applications: From field studies to laboratory research, our technology offers flexibility across various settings and disciplines.