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Tethered Liposomal Nanoparticle (tLN) Microarray Chips Containing Molecular Probes for Simultaneous Cell Sorting and Bio Detection

College of Engineering (COE)
Lee, L James
Kwak, Kwang Joo
Wu, Yun
Licensing Manager
Norris, Francis "Frank"

T2013-044 A simple, and efficient method for identifying intra-cellular biomarkers for the targeted treatment of disease.

The Need

Tethered Lipoplex Nanoparticle (TLN) microarray biochips simultaneously capture and identify extracellular vesicles (EV) RNA targets from human plasma or bodily fluids with or without EV isolation. Molecular detection probes, such as molecular beacons (MBs), identify specific micro RNA (miRNA) or messenger RNA (mRNA). Currently, there is an unmet need for simple and inexpensive methods of capturing and identifying targeted cells alive, especially in body fluids, for disease treatment.

The Technology

Researchers at The Ohio State University, led by Dr. L. James Lee, have developed a method that utilizes surface receptors to capture target cells from cell mixtures and identify specific mRNAs and miRNAs as intra-cellular biomarkers without damaging the cells. The microarray biochip consists of tethered liposomal nanoparticles that contain molecular probes, such as MBs, inside the particles and antibodies on the particle surface. The microarray chips can also be used to capture and identify virus and cell secreted nanoparticles, such as microvesicles and exosomes.

Commercial Applications

  • Liquid Biopsy
  • Stem Cell Sorting and Targeting
  • Virus Prevention and Detection


  • Simpler, less expensive, and easier to use than existing methods
  • Captures and identifies live cells
  • Captures and identifies targeted cells, virus, microvesicles, and exosomes in blood and body fluids.