The Need: Salmonella Dublin (S. Dublin) is of concern in the cattlepopulation. The current detection methods, particularly culture-based techniques are time-consuming and lack sensitivity in environmental samples. As a result, treatment effective surveillance and epidemiological understanding is hindered. There's a clear need for a culture-independent technique to efficiently detect Salmonella Dublin from clinical and environmental samples to enabling prompt identification of affected animals, premises and transmission pathways.

The Technology: We have developed a multiplex endpoint polymerase chain reaction (m-PCR) for the specific detection of Salmonella Dublin. This PCR method utilizes serovar-specific primers selected through rigorous assessments and provides high specificity and sensitivity. By targeting specific gene markers, our PCR technique offers a faster and more accurate means for the detection of Salmonella Dublin compared to traditional culture-based methods.

Stage of Development: Field trials conducted with this technology have demonstrated the enhanced performance of the developed m-PCR assay compared to traditional culture methods in detecting S. Dublin from environmental samples. By providing rapid and accurate detection, the m-PCR assay supports timely targeted interventions, contributing to better disease management and control in cattle environments, ultimately safeguarding animal and public health.

Commercial Applications:

• Surveillance and monitoring of Salmonella Dublin prevalence in dairy production environments.
• Rapid screening of environmental samples from calf production systems, including veal, dairy-beef, and calf dealer facilities.
• Quality control in food safety management systems to ensure compliance with regulatory standards.
• Reference lab use.

Benefits/Advantages:

• Improved sensitivity and specificity compared to culture-based methods, leading to enhanced detection..Rapid turnaround time, enabling timely interventions to prevent disease outbreaks and production losses.
• Cost-effectiveness through streamlined sample processing and reduced labor requirements.
• Facilitation of data-driven decision-making for disease management and control strategies.
• Enhanced understanding of Salmonella Dublin transmission, contributing to overall disease prevention efforts in both animal and human populations.